Biosenta's Tri-Filler(TM) Nanoparticles Achieve 100% Killing of Pathogens at Industry-Low Dose in Peer-Reviewed Studies

Validated University of Calgary research demonstrates broad-spectrum efficacy of core-shell calcium hydroxide-calcium carbonate (CSCC(TM)) particles

Toronto, Ontario and Calgary, Alberta--(Newsfile Corp. - June 11, 2025) - Biosenta Inc. (CSE: ZRO) ("Biosenta") today announced that two independent, peer-reviewed studies conducted at the University of Calgary confirmed that its proprietary Tri-Filler™ core-shell calcium hydroxide-calcium carbonate (CSCC™) nanoparticles achieved complete (100 %) bactericidal activity against multiple World Health Organization (WHO) priority pathogens and keep working far longer than conventional biocides.

"Transforming a readily available mineral into a durable, broad-spectrum antimicrobial opens new opportunities for safer surfaces in hospitals, transit systems and homes," said Dr. Mehdi Mohammadi Ashani, lead author of both papers.

Key laboratory findings

The research, conducted and independently validated by University of Calgary scientists, is detailed in two recent peer-reviewed publications in Colloids and Surfaces A and The Canadian Journal of Chemical Engineering.¹ ²

These studies confirmed Tri-Filler's™ potent efficacy against multiple World Health Organization priority bacterial pathogens, including:

• Gram-negative bacteria: Escherichia coli (E. coli), Pseudomonas aeruginosa
• Gram-positive bacteria: Staphylococcus aureus (including MRSA)

These findings were validated using both Minimum Inhibitory Concentration (MIC) and Minimum Biocidal Concentration (MBC) protocols, with consistent 100% elimination rates at the 2.5 mg/mL threshold across these bacterial strains. Traditional biocides rely on frequent re-application and can trigger resistance or toxicity. The 100 % kill rates reported above were obtained under controlled laboratory conditions.

In addition, Biosenta has obtained similar promising results against other significant microbes, including the Gram-positive bacterium Enterococcus faecalis and fungi Candida auris and Candida albicans. These results are currently under preparation for peer-reviewed publication.

"Achieving 100 per cent eradication across three WHO-priority species by a more environmentally friendly biocide at such relatively low dose moves Tri-Filler™ into a class of its own and is a decisive step toward safer, longer-lasting antimicrobial surfaces," added Dr. Maen Husein, professor of chemical and petroleum engineering and senior author of the papers.

From personal loss to public good

Biosenta's president and CEO Am Gill lost his uncle to a hospital-acquired infection. That tragedy drives the company's mission and underpins a $140-thousand donation to the Alberta Centre for Advanced Diagnostics to accelerate nanoparticle research.

"We're turning grief into action," Gill said. "Embedding Tri-Filler™ into concrete, paint and coatings will let us stop pathogens at the surface and save lives."

What happens next

• Regulatory filings - Health Canada and U.S. EPA submissions for Tri-Filler™-enabled products are in preparation.
• Manufacturing scale-up - Bench output already tops 170 kg day; full commercial plant design is under way.

About Biosenta Inc.

Biosenta Inc. develops innovative antimicrobial products that offer environmentally friendly solutions to a global market. Based in Toronto, Ontario and Calgary, Alberta, the company is dedicated to the advancement of technology that combats microbial growth in various materials. Biosenta's flagship product, Tri-Filler^®, represents a breakthrough in antimicrobial technology, providing durable protection against a wide spectrum of bacteria, molds, and fungi.

For more information, visit our website: www.biosenta.com

References

1. N. Darwish, M. M. Ashani, A. Mehairi, I. A. Lewis, M. M. Husein, "Synthesis of uniform core-shell calcium hydroxide-calcium carbonate biocidal particles via encapsulation into dry ice," Canadian Journal of Chemical Engineering, 2025, 1. DOI: 10.1002/cjce.25670.
   
2. N. Darwish, M. M. Ashani, I. A. Lewis, M. M. Husein, "Controlled carbonation of Ca(OH)₂ surface and its application as an antibacterial particle," Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2024, 682. DOI: 10.1016/j.colsurfa.2023.132852.

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